Arrayit Corporation Protein Microarrays

Protein Microarrays

The proteome encompasses the entire set of proteins produced by an organism. ArrayIt microarray technologies empower the production and analysis of a variety of microarrays including proteome microarrays. Target molecules are printed into microarrays using The Stealth Micro Spotting Device and NanoPrint or other Microarrayer and attached using a variety of surfaces depending on the application and detection method. The target samples printed on the microarray reacts with proteins, antibodies, drugs and receptors in solution that are incubated to the microarray. These protein microarrays can be made to be compatible with any microarray detection technique including standard fluorescence, SPR, chemiluminescence, colorimetric, RLS, mass spec and others.

Protein Microarray Applications include:

Expression Profiling
Serum Based Diagnostics
Protein-protein binding assays
Drug-target binding
Receptor epitope binding

ArrayIt microarray technologies can multiplex and miniaturize the analysis of tens of thousands of proteins for a variety of assays including drug binding, molecular interactions, enzyme activity, and pathway identification. Microarrays are excellent tools for discovering novel proteins that are key to understanding diseases and developing safe and effective drugs.

Pharmaceutical and biotechnology researchers use protein microarrays to streamline drug target identification, selection, validation, and predictive testing.

Figure 1: Libraries of express proteins are easily printed into microarrays with the NanoPrint Microarrayer. For detection of the printed proteins, samples can be directly labeled or a non-label detection technique can be used. A variety of protein- protein, protein-drug, and protein-small molecule interactions can be implemented. These microarrays can be used to analyze antibody specificity.

Figure 2: Our technologies are suitable miniaturizing and multiplexing assays that use both antigens and antibodies. We can implement MicroSpot ELISA (micro enzyme linked immunosorbent assay) and antibody microarrays. Detection can be colorimetric using secondary antibodies labeled with AP, HRP, biotinylated secondary antibodies that bind labeled streptavidin or direct fluorescent labeling. A variety of sandwich assays can be accomplished.

Figure 3: Our microarray technologies can be used to implement reverse phase microarrays. These microarrays are used to profile bound complex cell lysates, cells captured by LCM or serum samples printed into microarrays. The bound samples can be probed with antibodies for the detection of antigens present in the printed samples. Detection can be accomplished a variety of ways including use of a fluorescently labeled secondary antibody.

Figure 4: Synthetic proteins, peptides and engineered proteins to detect the presence of proteins in complex samples can also be implemented. Unique protein binding events, small molecule binding events or protein/protein interactions can be detected.